• Managed multiple sectors including Triagem, Coleta, Hematologia, Bioquímica, Imunologia, Microbiologia, Parasitologia, and Urologia.
• Conducted processing and collection of biological samples from children, adults, and elderly individuals.
• Performed tasks in Hematologia such as staining slides, differential cell counting, reticulocyte counting, TP, TTPA, VHS, complete blood count using semi-automatic equipment, and result interpretation.
• Executed research and identification of cysts, protozoa eggs in Parasitologia through slide preparation using Hoffman, lugol staining, Kato-Katz, Rugai, and Baermann-Moraes techniques.
• Conducted various immunoassays in Imunologia including rapid tests for HIV, PCR, Antigen Australia, Dengue, IgG-IgM, HCV, beta-HCG, Syphilis, and serial dilutions as needed.
• Prepared media, identified microorganisms using biochemical tests, conducted blood culture, urine culture, stool culture, and slide preparation and staining using gram and Ziehl Neelsen methods in Microbiologia.
• Analyzed physical, chemical, and microscopic properties of urine in Urologia, identifying structures such as crystals, cylinders, red blood cells, white blood cells, bacteria, and fungi.

• Executed extraction, purification, and quantification of DNA
• Conducted PCR and gel electrophoresis
• Utilized capillary electrophoresis and primer selection
• Analyzed molecular data to provide insights and recommendations.

• Proficiently managed multiple sectors including Triagem, Coleta, Hematologia, Bioquímica, Imunologia, Microbiologia, Parasitologia, Urologia, and Coleta Toxicológica.
• Demonstrated exceptional skills in overseeing various laboratory departments.
• Successfully performed duties in the areas of Coleta, Bioquímica, and Imunologia.
• Maintained high standards of quality control in Hematologia and Microbiologia.
• Conducted thorough analysis and testing in Parasitologia and Urologia.

• Conducted research in Microbiology, Immunology, Molecular Biology, Biochemistry, and Biotechnology.
• Performed isolation and identification of bacteria, biochemical and molecular tests, motility tests, microscopy, antibiograms, E-test, Vitek, MIC, Elisa, Western Blotting.
• Executed cell counting, seeding techniques, tissue culture, DNA extraction, multiplex PCR, LD50, histology.
• Contributed to the development of a vaccine against Caseous Lymphadenitis (Corynebacterium pseudotuberculosis) and the production of xanthan gum through the fermentative process of Xanthomonas campestris bacteria.
• Utilized batch bioreactor for fermentation process and conducted molecular characterization of Pequi (Caryocar brasiliense) using ISSR markers.

This work was performed as an integrated practical of a Biomedical Science undergraduate course of Biochemistry subject, in order to demonstrate used techniques to purify of Green Fluorescent Protein (GFP). To perform the experiments the main methodology applied was the by immobilized metal affinity chromatography (IMAC). The open reading frame for enhanced GFP was sub-cloned into the pQE30 expression vector. The subsequent production of protein tagged N-terminally with hexahistidine, facilitated its purification by IMAC. An approximate 3- fold purification of GFP was achieved. Thus, the students who completed the course gained significant experience related to fundamental techniques in molecular cloning and a sound basis in the principles of recombinant protein expression.